Coding

Part:BBa_K3286045:Design

Designed by: Sebastiaan Kuiper   Group: iGEM19_Wageningen_UR   (2019-10-16)


Monomeric red fluorescent protein (mRFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To assess whether we could inhibit bacteriophage lambda, we required the native promoter regions of bacteriophage lambda and fluorescent markers downstream of them.


Source

Bacteriophage lambda sequences were obtained from NCBI Reference Sequence: NC_001416.1 and synthetically synthesised in a gBlock. The RFP and GRP were ligated to the synthetic construct.

References