Composite

Part:BBa_K3286009

Designed by: Paul Alexander Niederau   Group: iGEM19_Wageningen_UR   (2019-10-16)


AcrIIA4 expression cassette

The AcrIIA4 is under control of the inducible L-rhamnose promoter. The AcrIIA4 expression was adapted by the use of different RBS sites.

The deeper analysis on varying Acr levels on inhibitory activity of dCas9 were assessed here: Part:BBa_K3286010

L-rhamnose inducible promoter: Part:BBa_K914003

AcrIIA4: Part:BBa_K3286000

bi-directional terminator: Part:BBa_K3286006


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None