Composite

Part:BBa_K3282007:Design

Designed by: Mateusz Piotr Szewczyk, Manjyot Kaur   Group: iGEM19_Lund   (2019-10-11)


PbrD and pbrT under Tac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1450
    Illegal NheI site found at 1648
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1398
    Illegal NgoMIV site found at 1861
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2005
    Illegal SapI.rc site found at 2235


Design Notes

Used Tac promoter to express the proteins in E. coli Nissle 1917


Source

The coding sequence is based on the lead binding protein K3282002 and lead transport protein K3282001. The Tac promoter K3282006 and Anderson ribosome binding site J61109 are used to express the proteins, followed by a TE terminators B0012.

References