Part:BBa_K3281014:Design

eGFP Coding Sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The actin localizing section of the eGFP sequence was cloned out in order to reduce the transcript length and improve efficiency of subsequent Gibson assemblies.

Source

This part was cloned from an eGFP-actin localizing reporter that Harvard University's Silver lab had in stock.