Reporter

Part:BBa_K3281009:Design

Designed by: Patrick Dickinson   Group: iGEM19_Harvard   (2019-10-21)


tTA (TANGO assay) Reporter - eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The actin-localizing portion of eGFP was removed in cloning as we had no particular reason to fluoresce just actin, and the resulting reduction in amplicon length made for more efficient Gibson assembly.


Source

tTA sequence was derived from an addgene plasmid, and the source DNA can be found at this address:

https://www.addgene.org/browse/sequence/204193/

eGFP was cloned from an eGFP-actin localizing reporter that was in stock at Harvard University's Silver Lab.

References