Coding
Part:BBa_K3279004:Design
Designed by: Shujie Liao Group: iGEM19_CAU_China (2019-10-14)
beta-carotene 3-hydroxylase (CrtZ)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 226
Illegal PstI site found at 436 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 514
Illegal PstI site found at 226
Illegal PstI site found at 436 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 226
Illegal PstI site found at 436 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 226
Illegal PstI site found at 436
Illegal AgeI site found at 211 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was synthesized by Sangon Biotech and the codon of this part was optimized to increase its translation rate in E. coli.
Source
The original sequence comes from Pantoea ananatis but we have codon optimized it.