Composite

Part:BBa_K3276005:Design

Designed by: Jackie Shane   Group: iGEM19_CSL_Pittsburgh   (2019-10-16)


Cytochrome P450 driven by a constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1180
    Illegal SapI.rc site found at 386


Design Notes

CYP6AY1 was codon optimized for expression in E. coli


Source

cytochrome P450 sequence was obtained from NCBI GenBank: CAH65682.2

References