Composite

Part:BBa_K3276003:Design

Designed by: Jackie Shane   Group: iGEM19_CSL_Pittsburgh   (2019-10-16)


Cytochrome P450 with pelB secretion signal and 6xHis tag reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 125
    Illegal AgeI site found at 551
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CYP6AY1 had to be optimized for E. coli expression by removing the introns and IDT's codon optimization software


Source

Part:BBa_S05355 contains the constitutive promoter J23119 with RBS B0034 CYP6AY1 was optimized from NCBI sequence GenBank: CAH65682.2

References