Plasmid

Part:BBa_K3265029:Design

Designed by: Roy Hove   Group: iGEM19_UZurich   (2019-10-18)


gp88-egfp (Phage201-phi2-1 protein fusion to egfp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 139
    Illegal NotI site found at 1633
    Illegal NotI site found at 4221
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2438
    Illegal BglII site found at 2899
    Illegal BglII site found at 3674
    Illegal BamHI site found at 1658
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Choosing which terminus to put the fluorescent protein.


Source

Phage Genomic DNA

References