Coding

Part:BBa_K3265027:Design

Designed by: Alexander Schanne   Group: iGEM19_UZurich   (2019-10-15)


gp105-GFP fusion (Phage 201 phi2-1 main shell protein gp105)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2616
    Illegal NotI site found at 139
    Illegal NotI site found at 1633
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2672
    Illegal BamHI site found at 1658
    Illegal XhoI site found at 3840
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2556
    Illegal AgeI site found at 3849
    Illegal AgeI site found at 4068
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3270


Design Notes

egfp makes for a very good signal in the bacteria, originally we used YFP which gave a very weak signal.



Source

phage 201 phi2-1 DNA, egfp from plasmid. assembled via conventional cloning.

References