Device

Part:BBa_K3264024:Design

Designed by: Diol Wang   Group: iGEM19_GreatBay_SZ   (2019-10-16)


TALEsp-vioA-vioB-vioC-vioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1513
    Illegal NheI site found at 1921
    Illegal NheI site found at 4184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 113
    Illegal XhoI site found at 2152
    Illegal XhoI site found at 2692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6966
    Illegal AgeI site found at 747
    Illegal AgeI site found at 849
    Illegal AgeI site found at 1767
    Illegal AgeI site found at 2277
    Illegal AgeI site found at 7162
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A TALE stabilized "constitutive" promoter generate enzyme vioA, vioB, vioC, and vioE. This part is working on a low copy number pSC101 plasmid.


Source

Composite part, sources are come from iGEM part collection.

References