Generator

Part:BBa_K325905:Design

Designed by: Will Handley and Emily Knott   Group: iGEM10_Cambridge   (2010-10-23)

Bacterial Lux reporter
CDEG pλ AB
(E. coli optimised)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3112
    Illegal AgeI site found at 3327
    Illegal AgeI site found at 3915
    Illegal AgeI site found at 4898
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5417


Design Notes

This part is a PoPS->light device that needs to be placed under a promoter and induced. It has been assembled from the parts LuxCD (BBa_K325902), LuxEG (BBa_K325903), a RiPS generator using plambda (BBa_K081006) and LuxAB (BBa_K216008) using the method of Gibson Assembly. Therefore no BioBrick scars have been created.


Source

a

References