Plasmid_Backbone
MP
Part:BBa_K3258044
Designed by: Alec Lourenco Group: iGEM19_Stanford (2019-10-21)
Plasmid that encodes an arabinose-inducible mutagenesis cassette to increase mutation rate for use in PACE or PREDCEL+.
When uninduced, E. coli mutation rate is 2.38E-‐10 mutations per base pair and 5.09E-‐07 per base pair when induced.
Use 25 mM arabinose to induce the cassette. Use 25 mM glucose to repress mutagenesis.
Genes in the mutagenesis cassette used to disrupt efficient DNA replication include:
- BR1 (proofreading)
- dam (methyl-directed mismatch repair)
- seqA (methyl-directed mismatch repair)
- emrR (base selection)
- ugi (base excision repair)
- CDA1 (base excision repair)
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Parameters
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