Composite

Part:BBa_K3258032:Design

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-21)


MDV + GFP Flipped


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 146
    Illegal NotI site found at 1118
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
    Illegal BglII site found at 1133
    Illegal XhoI site found at 168
    Illegal XhoI site found at 1127
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1136
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 438


Design Notes

Constructed as an in vivo and in vitro indicator of the functioning of Qbeta in a system.


Source

Synthetic construct.

References