RNA

Part:BBa_K3258022:Design

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-20)


Scr Scramble Non-targeting gRNA Negative Control


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 30
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 226
    Illegal BsaI site found at 404


Design Notes

Designed to function as a negative control for CRISPR Cas9 RFP knockdown experiments with Anti-CRISPR.


Source

Synthetic construct derived from Dr. Stanley Qi's lab.

References