Coding

Part:BBa_K3258020:Design

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-20)


Ampicillin resistance gene with 1 stop codon point mutation, A79T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Point mutation to create early stop codon to result in non-functioning ampicillin resistance gene.


Source

Part derived originally from standard ampicillin resistance gene for E. coli.

References