Composite
Part:BBa_K3257119
Designed by: Yi Qiao Group: iGEM19_Fudan-TSI (2019-10-16)
lox5171-truncated mCherry-loxP
This part is a truncated mCherry fused two lox sites on both sides. It is used for the verification of the homologous recombination conducted by Cre. We purposely have not added a promoter or terminator in this composite part because, after homologous recombination, a wild-type mCherry will be recombined into this locus and we will be unable to detect red fluorescence since wild-type mCherry cannot be expressed at all.
Prove of well-functional mCherry
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 392
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 392
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 392
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 392
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |