Coding

Part:BBa_K3257032:Design

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-04)


EGFP_K159X


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is similar to the wild type EGFP except that it does not give out green light as the wild type EGFP does. We mutate BBa_S03452 475nd nucleotide from A to T to make a stop codon at 159th amino acid residual.

Source

Mutated from BBa_S03452

References