Part:BBa_K3257005
Added by LZU-HS-Pro-B
Build Stage
We obtained the gene fragments we designed through DNA synthesis.
-First, the plasmid was extracted for PCR amplification. Then double digestion of PCR fragments and plasmids, nucleic acid electrophoresis, and gel recovery experiment were carried out.
-The PCR fragments and plasmids digested successfully were recovered. The target gene and plasmid were linked to transforming the linked product.
-After the screening, the plasmid was successfully constructed.
-Prepare receptor cells, and transform the engineering vector into the expression host E.coli BL21.
Test Stage
The red line in the middle shows the effect of the LacUV5 promoter ligating the red fluorescent protein.
For the experimental results, it was evidence presented that the perceptive rate of uric acid greatly increased after we added the amplification system into our experiment. We have discovered and concluded the general rule that the amplification system we designed and added was able to amplify the signal by a factor of four, which enabled us to better sense and detect the amount of uric acid in our bodies.
LacUV5 Promoter
E.coli lac promoter with an /"up/" mutation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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