Composite
sfGFPTLU

Part:BBa_K3254024

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-16)


RiboJ-B0034-sfgfp

This is a superfold-GFP translational unit. The RiboJ can insulate the interference by different 5'-UTR sequence. As a result, the sfGFP expression amount can be used for calibrating the transcriptional activity of its upstream sequence without considering the different translational rate caused by the upstream RNA sequence.

Usage

  • This part can be used for quantitative testing the characteristics of transcriptional regulation device.
  • The Ptac-sfGFP is a good example for such testing.
  • You can see the page of BBa_K3254025 for getting more information.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 114


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Categories
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Parameters
None