Coding
INT5

Part:BBa_K3254006:Design

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-09)


Integrase Int5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 30
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 22
    Illegal AgeI site found at 406
    Illegal AgeI site found at 781
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was first reported in 2014. Two PstI site were eleminated by synonymous mutations.


Source

The original organism is the Streptomyces phage PhiK38-1. The DNA was coloned from the source plasmid(pCis2+7+8+5, see reference).

References

Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6.