Plasmid

Part:BBa_K3253005:Design

Designed by: HAOYU JIANG   Group: iGEM19_Nanjing   (2019-10-15)


pCAMBIA2301


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 10821
    Illegal XbaI site found at 10848
    Illegal PstI site found at 10860
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10821
    Illegal NheI site found at 2025
    Illegal NheI site found at 5470
    Illegal PstI site found at 10860
    Illegal NotI site found at 3344
    Illegal NotI site found at 4877
    Illegal NotI site found at 6167
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10821
    Illegal BglII site found at 7
    Illegal BglII site found at 9778
    Illegal BamHI site found at 10842
    Illegal XhoI site found at 8913
    Illegal XhoI site found at 9791
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 10821
    Illegal XbaI site found at 10848
    Illegal PstI site found at 10860
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 10821
    Illegal XbaI site found at 10848
    Illegal PstI site found at 10860
    Illegal NgoMIV site found at 2789
    Illegal NgoMIV site found at 2963
    Illegal NgoMIV site found at 3013
    Illegal NgoMIV site found at 3017
    Illegal NgoMIV site found at 3044
    Illegal NgoMIV site found at 3543
    Illegal NgoMIV site found at 3901
    Illegal AgeI site found at 5964
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5867
    Illegal SapI site found at 6558
    Illegal SapI site found at 9070
    Illegal SapI site found at 9280
    Illegal SapI.rc site found at 6095


Design Notes

It contains multiple restriction sites and we will use EcorI and HindIII site.


Source

We purchased this part from takara company.

References