Primer

Part:BBa_K3253001:Design

Designed by: HAOYU JIANG   Group: iGEM19_Nanjing   (2019-10-12)


Gene-EcoR1-F


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add protective bases on the sequence to protect it in restriction enzyme's cutting process


Source

it is a primer which corelates with the enzyme EcoR1-F

References