Composite

Part:BBa_K3251000

Designed by: Rachel Lee   Group: iGEM19_Michigan   (2019-10-14)


IPTG-induced LuxR expression

This part is an improvement of Biobrick BBa_F2621, consisting of the luxR protein coding region, as well as the luxpR promoter which can be activated by the binding of luxR+3OC6HSL complex. Part BBa_F2621 was shown to be defective since it lacks the BBa_R0063 promoter (according to iGEM11_Wageningen). We added an inducible IPTG promoter upstream to the luxR coding region and a yellow chromoprotein downstream of the luxR gene. This new part construct allows the inducible expression of luxR by IPTG with a visualization by the yellow reporter chromoprotein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1710


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