Device

Part:BBa_K3244029:Design

Designed by: Mohammad Tarek Mansour   Group: iGEM19_AFCM-Egypt   (2019-10-10)


CRISPR HDR for IL-18-producing TRUCK CAR-T Cell


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3771
    Illegal XhoI site found at 808
    Illegal XhoI site found at 1630
    Illegal XhoI site found at 2454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3146
    Illegal NgoMIV site found at 6505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6175
    Illegal BsaI.rc site found at 298
    Illegal BsaI.rc site found at 5872
    Illegal SapI site found at 6371


Design Notes

NA


Source

IDT

References