Part:BBa_K3244029
CRISPR HDR for IL-18-producing TRUCK CAR-T Cell
[http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have added Part BBa_K3244027 which represents a composite device of 2nd Generation CAR-T, part BBa_K3244028 which represents a composite IL-18 expression construct for TRUCK CAR design and finally BBa_K3244029 which represents CRISPR Homology Directed Repair template with both L-Arm and R-Arm for IL-18-producing TRUCK CAR-T Cell. Results of functional characterization: A statistical significance difference was achieved between the % of viability in CAR-T treated BC cells associated Schistosomiasis and unassociated cells (p<0.05). Moreover, between untreated and CAR-t treated BC cells (p<0.01). High reduction of viable BC cells that was treated by CAR-T was demonstrated in Schistosomiasis associated BC compared to unassociated cells, but no statistical significance was achieved (p>0.05).BC cells that was treated with CAR-t cells engineered for ERB2 and Schistosoma egg antigen should a significant apoptosis (54.8%) and 21% dead tumor cells.
For results of functional characterization please refer to our parts Page EGYPT-AFCM 2019 Parts Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3771
Illegal XhoI site found at 808
Illegal XhoI site found at 1630
Illegal XhoI site found at 2454 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3146
Illegal NgoMIV site found at 6505 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6175
Illegal BsaI.rc site found at 298
Illegal BsaI.rc site found at 5872
Illegal SapI site found at 6371
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