Composite

Part:BBa_K324003:Design

Designed by: Julius Ho   Group: iGEM10_Brown   (2010-10-25)


TetR repressible generator of LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These parts were joined together with standard biobrick assembly, even though the LacI protein was in RFC25 and thus capable of protein fusion assembly as well.


Source

A simple Biobrick assembly was conducted to join the TetR promoter+RBS (BBa_J13002) with LacI (BBa_K324000). J13002 was obtained from the 2010 Spring Distribution, while K324000 was adapted by 2010 Brown iGEM from an existing part. See the part's page for further details

https://parts.igem.org/Part:BBa_J13002 https://parts.igem.org/Part:BBa_K324000

References