Protein_Domain

Part:BBa_K324002:Design

Designed by: Julius Ho   Group: iGEM10_Brown   (2010-10-25)

Tat protein transduction domain with glycine linker (RFC25)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RFC25 fusion assembly format was used because this protein domain is intended to be fused to other proteins to convey its membrane-crossing characteristics. N terminal and/or C terminal addition of the Tat domain have both been shown to be effective.


Source

The sequence originates from the Tat protein in human immunodeficiency virus. We obtained the DNA from Will Donovan (william_donovan@brown.edu)

References