Regulatory

Part:BBa_K3239005:Design

Designed by: Xingyu Zhou   Group: iGEM19_ShanghaiFLS_China   (2019-10-15)


Prm1 transcription factor in the P.pastoris GS115 strain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1034
    Illegal PstI site found at 1556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1034
    Illegal NheI site found at 2815
    Illegal PstI site found at 1556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1034
    Illegal BglII site found at 387
    Illegal BamHI site found at 1917
    Illegal BamHI site found at 2836
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1034
    Illegal PstI site found at 1556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1034
    Illegal PstI site found at 1556
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleotide 2811 (A) is changed to (G) for Type IIS compatiblility.

Source

P. pastoris GS115

References