Regulatory

Part:BBa_K3239002:Design

Designed by: Xingyu Zhou   Group: iGEM19_ShanghaiFLS_China   (2019-10-15)


Promoter for the Mit1 transcription factor in the P.pastoris GS115 strain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 793
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleotide 428 (A) was changed to (T) for Type IIS compatibility.

Source

P. pastoris GS115

References