Composite

Part:BBa_K3237029:Design

Designed by: Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-16)


nucA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

not codon optimized. No changes to the sequence was done as the implementation of this gene would have been for it to be placed within the genome. Is a part of a kill switch designed by Lethbridge iGEM 2019. Terminator used was BBa_B0015 as used in the literature [1].


Source

Genbank X64706.1 Anabaena sp. nucA gene for nuclease.

References

1.Čelešnik, H., Tanšek, A., Tahirović, A., Vižintin, A,. Mustar, J., Vidmar, V., and Dolinar, M. (2016) Biosafety of biotechnologically important microalgae: intrinsicsuicide switch implementation in cyanobacteriumSynechocystissp.PCC 6803. The Company of Biologists. 5, 519-528.

2.Lindbland, P., Fuene, D., Borbe, F., et al. (2019) CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels. Algal research. 41, https://doi.org/10.1016/j.algal.2019.101510