Composite

Part:BBa_K3237016:Design

Designed by: Aubrey Demchuk, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-05)


His-mRFP1-Proinsulin with Furin cut sites Codon Optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 649
    Illegal AgeI site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Protein linkers were placed after the Furin cut sites to allow proper folding during expression. This part was also codon optimized for E.coli .

Source

This part is based on the sequences from BBa_K1328003 and BBa_E1010. The part was synthesized by idt.


References

1. Hay, C., and Docherty, K. (2003) Enhanced expression of a furin-cleavable proinsulin. Journal of molecular endocrinology. 3, 597-607.