Composite

Part:BBa_K3237015:Design

Designed by: Aubrey Demchuk, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-05)


His-mRFP1 codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1502
    Illegal XbaI site found at 1490
    Illegal XbaI site found at 1532
    Illegal SpeI site found at 1526
    Illegal PstI site found at 1040
    Illegal PstI site found at 1508
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1502
    Illegal NheI site found at 1054
    Illegal SpeI site found at 1526
    Illegal PstI site found at 1040
    Illegal PstI site found at 1508
    Illegal NotI site found at 1538
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1502
    Illegal BamHI site found at 1520
    Illegal XhoI site found at 656
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1502
    Illegal XbaI site found at 1490
    Illegal XbaI site found at 1532
    Illegal SpeI site found at 1526
    Illegal PstI site found at 1040
    Illegal PstI site found at 1508
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1502
    Illegal XbaI site found at 1490
    Illegal XbaI site found at 1532
    Illegal SpeI site found at 1526
    Illegal PstI site found at 1040
    Illegal PstI site found at 1508
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After the N-terminal histag we also have added a GC linker so that the his tag addition will not affect the protein structure of mRFP1.

Source

The part is based on the part BBa_E1010 in the iGEM registry. The part was codon optimized and then synthesized by TWIST.

References