Part:BBa_K3237013:Design

Insulin Receptor Protein under Constitutive Expression

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The insulin protein has glycosylation sites that help form the protein [2]. Therefore buffer modifications or processing during purification modifications should be considered. In terms of design, nothing was modified in order to emulate human receptors in the best way possibles with the exception of the Histidine tag to provide more efficient purification and codon optimized for E.coli .

Source

This part was taken from uniprot.org accession number P08069. The part was synthesized and purchased from IDT.

References

1. Lee, J., and Pilch, p. (1994) The insulin receptor: structure, function and signaling. American Journal of Physiology. 266:319-334.

2. Garret T., McKern, N., Lou, M., Frenkel, M., Bently, J., Lovrecz, G., Elleman, T., Cosgrove, L., and Ward, C. (1998) Crystal structure of the first three domains of the type-1 insulin-like growth factor receptor. Nature. 394: 395-399.