Coding

Part:BBa_K3237004:Design

Designed by: Aubrey Demchuk, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-11)


His-mRFP1-Proinsulin with furin cut sites codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 363
    Illegal XhoI site found at 904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An extra furin cut site and linker additions were added to provide further processing. The mRFP1 was added as a part of our expression standardization strategy.

Source

This part is an improved part from Tsinhua 2014. The mRFp1 sequence is from the registry (BBa_E1010). The part was synthesized by IDT.

References

1. Hay, C., and Docherty, K. (2003) Enhanced expression of a furin-cleavable proinsulin. Journal of molecular endocrinology. 3, 597-607.