Generator

Part:BBa_K323138:Design

Designed by: Mattia Petroni   Group: iGEM10_Slovenia   (2010-10-20)

R0011 crtEBIYO

Carotenoid biosynthesis operon. Comprises of beta-carotene biosynthesis operon from P. ananatis and synthetic crtO (beta carotene ketolase) gene with Elowitz RBS. The operon is placed under lacI promoter (Bba_r0011) Presumably synthesises echinenon (not confirmed). For canthaxanthin biosynthesis crtO overexpression is required.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5708
    Illegal BamHI site found at 2037
    Illegal BamHI site found at 5834
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1573
    Illegal NgoMIV site found at 1703
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

a


Source

a

References