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Part:BBa_K323021:Design

Designed by: Jernej Turn�ek   Group: iGEM10_Slovenia   (2010-10-20)

CMV ATG Gli1 link nCFP stop - part of mammalian split/FRET system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Cyan fluorescent protein (CFP) was obtained from a commercially available vectror C1. Gli-1 sequence was already deposited in a Registry as BBa_K165007.

References

Kerppola T.K., Hu C. 2003. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nature biotechnology (21): 539-545

Nurmemmedov E, Thunnissen M. 2006. Expression, purification, and characterization of the 4 zinc finger region of human tumor suppressor WT1. Protein Expr Purif.;46 (2): 379-89