Coding

Part:BBa_K3228100:Design

Designed by: Marian Krämer   Group: iGEM19_Marburg   (2019-10-18)


pMC_0_4_35_cpf1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1713
    Illegal NheI site found at 3433
    Illegal SpeI site found at 3622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was aquired via PCR with primers that have BsmbI cutting sites as overhang. The overhangs were chosen to anneal to the backbone of the digested Lvl0 entry vector.

Source

The part was aquired via PCR from the vector pSL2680, which derived from RSF1010.

References