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Part:BBa_K3228001:Design

Designed by: Maurice Mager   Group: iGEM19_Marburg   (2019-09-25)


pMC_0_1_23_aNSo2_integration (neutral integration site for cyanobacteria)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Regions were determined from transcriptomic data to not have any transcription activity from neighbouring regions as well as not containing any internal BsmbI or BsaI cutting sites. As a connector/homology part, this part naturally contains an internal BsaI site for Lvl2 MoClo cloning in the Marburg Collection.


Source

Part derived from genomic DNA of S. elongatus UTEX2973 via PCR.

References