Other
Part:BBa_K3228000:Design
Designed by: Maurice Mager Group: iGEM19_Marburg (2019-09-25)
pMC_0_1_22_aNSo1_integration (neutral integration site for cyanobacteria)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 228
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Regions were determined from transcriptomic data to not have any transcription activity from neighbouring regions as well as not containing any internal BsmbI or BsaI cutting sites. As a connector/homology part, this part naturally contains an internal BsaI site for Lvl2 MoClo cloning in the Marburg Collection.
Source
Part derived from genomic DNA of S. elongatus UTEX2973 via PCR.