Coding

Part:BBa_K3226019

Designed by: Yuma matsumoto   Group: iGEM19_Botchan_Lab_Tokyo   (2019-10-16)


RecA+his

<Long description>

This coding sequence has 6×histidine (BBa_K2888600) attached to RecA was designed by iGEM11_Osaka. This coding sequence is needed to make device (BBa_K3226020). The following sentences are mainly related to RecA.

Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions.

<Usage>

This is mainly used to obtain resistance to radiation. In our project, we used γ-rays as one type of radiation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 242
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 916
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None