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Composite
RecA(Devic

Part:BBa_K3226000:Design

Designed by: Yuma Matsumoto   Group: iGEM19_Botchan_Lab_Tokyo   (2019-10-14)


This is a device, in which conducts an operation in the cell. This can express the RecA gene.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2029
    Illegal XbaI site found at 2044
    Illegal SpeI site found at 3372
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2029
    Illegal SpeI site found at 3372
    Illegal NotI site found at 2035
    Illegal NotI site found at 3379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2029
    Illegal BglII site found at 2499
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2029
    Illegal SpeI site found at 3372
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2029
    Illegal XbaI site found at 2044
    Illegal SpeI site found at 3372
    Illegal NgoMIV site found at 3173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We make this device by using in-fusion cloning. We added homologous sequence between insert and vector by PCR because the primers are needed that sequence in in-fusion cloning.


Source

Deinococcus radiodurans

References

・In-Fusion® HD Cloning Kit User Manual., Takara Bio USA, Inc., p.3-4.

・Dea Slade1,and Miroslav Radman,Oxidative Stress Resistance in Deinococcus radiodurans, Microbiol Mol Biol Rev. 2011 Mar; 75(1): 133–191.