Deleted
Sample Discontinued
Experience: None
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Composite
RecA(Devic

Part:BBa_K3226000

Designed by: Yuma Matsumoto   Group: iGEM19_Botchan_Lab_Tokyo   (2019-10-14)


This is a device, in which conducts an operation in the cell. This can express the RecA gene.

Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2029
    Illegal XbaI site found at 2044
    Illegal SpeI site found at 3372
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2029
    Illegal SpeI site found at 3372
    Illegal NotI site found at 2035
    Illegal NotI site found at 3379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2029
    Illegal BglII site found at 2499
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2029
    Illegal SpeI site found at 3372
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2029
    Illegal XbaI site found at 2044
    Illegal SpeI site found at 3372
    Illegal NgoMIV site found at 3173
  • 1000
    COMPATIBLE WITH RFC[1000]


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