Coding

Part:BBa_K3225005:Design

Designed by: Yaran Zhao   Group: iGEM19_iBowu-China   (2019-10-21)


ExpR71, transcriptional factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 717
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 249


Design Notes

The codon of ExpR was modified for E.coli.BioBrick RFC10 assembly method could be used.



Source

De-novo synthesis


References