Translational_Unit

Part:BBa_K322451

Designed by: William Rostain   Group: iGEM10_Edinburgh   (2010-09-01)

Photinus pyralis luciferase mutated for increased bioluminescence.

Mutations Ile423Leu Asp436Gly and Leu530Arg increase the bioluminescence intensity by a factor of 12.5 according to Fujii et al. (2007). It is codon optimised for expression in E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1417
    Illegal NgoMIV site found at 1458
    Illegal AgeI site found at 1150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None