Composite

Part:BBa_K322247

Designed by: William Rostain   Group: iGEM10_Edinburgh   (2010-10-08)

This part is made of the lac promoter followed by S284T firefly luciferase mutant, which shifts its emission spectrum to make it red. Note that firefly luciferase is temperature sensitive, and cells grown at 30°C are considerably brighter than cells grown at 37°C.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


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