Translational_Unit
Part:BBa_K322210:Experience
Designed by: Geraldine Avila Group: iGEM10_Edinburgh (2010-09-25)
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Applications of BBa_K322210
Characterisation of the activity of the cat BioBrick:
Cell lines containing cat in pSB1A2 (red boxes) grew on both ampicillin (100mg/ml) chloramphenicol (40mg/ml) whereas cell lines containing a pSB1A2 control (Edinbrick, blue boxes) only grew on ampicillin (100mg/ml). This experiment confirms that cat is effective as a negative selection marker within the BRIDGE protocol.
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