Translational_Unit

Part:BBa_K322210:Design

Designed by: Geraldine Avila   Group: iGEM10_Edinburgh   (2010-09-25)

Chloramphenicol acetyltransferase (cat)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A considerable amount of upstream DNA was included in the hope that this would include the promoter region.

The PCR product, P1601, was initially cloned in pSB1A2, which made it easy to confirm that the gene was active, since the transformants became chloramphenicol resistant. In the course of the iGEM project, the same PCR product was cloned in pSB1C3 to comply with new submission rules. Since this BioBrick was originally prepared for internal use, it uses abbreviated forms of the prefix and suffix lacking the NotI sites, but is in all ways compatible with RFC10 assembly.

Source

Source: plasmid pTG262 (broad host range vector for Gram positive and Gram negative bacteria).


References