Generator

Part:BBa_K3222005

Designed by: Ryo NIWA   Group: iGEM19_GIFU_TOKAI   (2019-11-01)


sfGFP generator (Lack of 3

Group: GIFU TOKAI 2019

Author: Ryo NIWA


Documentation:

This part was made to use as control for iGEM GIFU TOKAI 2019's project. Since this part does not have 3' UTR region, it is low-expression and you cannot confirm sfGFP as fluorescence. We confirmed this part is functional by performing Western Blotting. When this part is circularized, this part may be possible to produce high amount of sfGFP


T--GIFU_TOKAI--western_last.png

Figure.1 Western Blotting


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 75


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Categories
Parameters
None