Composite

Part:BBa_K3221203:Design

Designed by: Hao Zhou   Group: iGEM19_SCU-China   (2019-10-04)


pMET3-GAL4-CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 302
    Illegal XhoI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 399
    Illegal BsaI site found at 637
    Illegal BsaI.rc site found at 2313
    Illegal SapI site found at 1002


Design Notes

We designed this part to fit the RFC10.


Source

We got the pMET3 and the GAL4 from the BY4742 genomic. And the CYC1 terminator is initially on the pYES2.

References